Translational_Unit

Part:BBa_K1159116:Design

Designed by: Dong-Jiunn Jeffery TRUONG   Group: iGEM13_TU-Munich   (2013-09-16)


Eukaryotic light-switchable TEV Protease translational unit (PhyB/PIF6 based) with downstream IRES


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 487
    Illegal BamHI site found at 569
    Illegal BamHI site found at 3432
    Illegal BamHI site found at 4862
    Illegal XhoI site found at 520
    Illegal XhoI site found at 539
    Illegal XhoI site found at 2674
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3849
    Illegal AgeI site found at 3196
    Illegal AgeI site found at 3551
    Illegal AgeI site found at 4626
    Illegal AgeI site found at 4981
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2059
    Illegal BsaI site found at 2465
    Illegal SapI site found at 736
    Illegal SapI.rc site found at 3151
    Illegal SapI.rc site found at 3629
    Illegal SapI.rc site found at 4170
    Illegal SapI.rc site found at 5059


Design Notes

x


Source

Synthetic

References